GeoMx Digital Spatial Profiler and nCounter

Concepts

• nanostring geomx
• geomx digital spatial profiler
• cardiff
• analysis apparatus
• novel digital technology
• geomx dsp
• ncounter instruments
• ncounter
• nucleic acids
• proteins

Location

Cardiff and Vale of Glamorgan:

Description

NanoString GeoMX and nCounter instruments

Total Quantity or Scope

The GeoMx DSP is a novel digital technology (proprietary to NanoString) that is based on multiplexed measurement of protein and nucleic acids and offers unparalleled levels of precision coupled with the ability to quantify up to 96 proteins and over 1000 RNA targets on a formalin-fixed, paraffinembedded (FFPE) tissue section on a microscope slide.

Award Detail

CPV Codes

• 38432000 - Analysis apparatus

Legal Justification

The GeoMx Digital Spatial Profiler (DSP) is only available from NanoString Technologies, Inc. The GeoMx DSP is a novel digital technology (proprietary to NanoString) that is based on multiplexed measurement of protein and nucleic acids and offers unparalleled levels of precision coupled with the ability to quantify up to 96 proteins and over 1000 RNA targets on a formalin-fixed, paraffinembedded (FFPE) tissue section on a microscope slide. In contrast to the sequential analysis of multi-target immunohistochemistry (IHC) slides, the GeoMx DSP samples all protein or RNA analytes on a single slide. This not only shortens experiments and simplifies data analysis, but also provides a higher multiplexing capacity (96 targets) all with spatial context from Formalin-Fixed, Paraffin-Embedded (FFPE) tissue sections. Based on NanoString’s proprietary digital barcoding technology, the GeoMx DSP platform measures local protein levels, and can be combined with RNA expression, within heterogeneous tissue samples. Combining both multiplexed nucleic acid and protein on the same platform gives researchers the ability to spatially resolve RNA when suitable antibodies do not exist. The GeoMx DSP assay is performed on the GeoMx DSP platform which includes imaging and fluidic components to capture spatial context, and current nCounter® or next-generation sequencing (NGS) instruments provide the quantification. Protein detection is enabled via primary antibodies which are covalently attached via a UV photocleavable linker to DNA indexing oligos. Following antigen retrieval, FFPE tissue samples are stained with a multiplexed cocktail of labeled antibodies, and DNA oligos are subsequently released by UV light exposure across regions of interest. The liberated DNA oligos are then hybridized to optical barcodes for quantitation on an nCounter or NGS instrument. This technique enables quantitative, multiplexed protein detection up to 5.5 logs of dynamic range. Key Features and Benefits of DSP technology - Multiplex many analytes on one tissue section in a single pass - Quantitation based on linear single-molecule counting: up to 5.5 logs - Single-cell limit of detection - Non-destructive: sample completely intact after assay - Single antigen retrieval without effects from order-of-addition

Other Information

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