UKRI-2399 High Specification Flow Cytometer Cell Sorter (minimum 6 lasers)

Concepts

• cytometers
• swindon
• flow cytometer cell sorter
• cell types
• lmb flow cytometry unit wish
• communal cell sorter
• high specification
• purification of sensitive cell types
• high purity
• single-cell organoid suspensions

Location

Cambridgeshire CC

Description

The LMB Flow Cytometry Unit wish to purchase a high specification, high throughput flow cytometer cell sorter with a minimum of 6 spatially separated lasers and a minimum of 30 fluorescence channels that is housed under a certified Class II biocontainment hood. The flow cytometer cell sorter should provide increased resolution and flexibility to sort a wide range of particles, bacteria and cell types (both primary and cultured) in various formats including 4 way or 6-way sorting, and in 96 and 384 well formats. The goal is to provide a communal cell sorter to support research in areas of artificial biology requiring chromosome sorting for the rewriting of the human genome. Customizable options in nozzle size and pressure settings will allow purification of sensitive cell types such as neurons and single-cell organoid suspensions, facilitating downstream functional experimentation.

Total Quantity or Scope

The LMB Flow Cytometry Unit intend to purchase and install a high specification flow cytometer cell sorter, equipped with a high throughput plate sorting option, housed under a certified Class II microbiological safety hood and operating a minimum of 6 different wavelength spatially separated lasers in order to perform purification and fractionation of various cell types and particles utilising a broad range of fluorescent markers. Objectives 1.The cell sorter should be equipped with 6 high-powered, spatially separated lasers with the following excitation wavelengths and powers; 349-355nm (100mW), 405nm (100mW), 445nm (100mW), 488nm (100mW), 561nm (100mW), 637-640nm (100mW). 2.The cell sorter should be housed under a certified Class II biocontainment system. 3.The cell sorter should be outfitted with a fluorescence unmixing and/or compensation algorithm to separate and correct for spectral overlap of individual signatures of highly overlapping fluorochromes or fluorescent proteins. 4.The cell sorter should be capable of achieving a signal processing rate of 70,000 events per second (eps) for analysis and the ability to sort particles at high purity (≥99%) at flow rates ≥20,000 particles per second. 5.The cell sorter should be able to detect and distinguish small particles from noise using the side scatter detector (SSC) as low as 200nm. 6.The cell sorter should be outfitted with a high-throughput plate option, capable of sorting single cells/particles in a wide range of plate formats including customized, 6, 12, 24, 96, and 384 well. 7.The cell sorter should be upgradable to include additional lasers such as 375nm, 532nm, 594nm, 795-808nm. 8.The cell sorter should come equipped with a temperature control option for both the sample input and collection device from 4-42oC. Maintenance contract (parts and labour) should be detailed in years 3, 4 and 5 after successful installation following expiry of the requested 2-year warranty period. Additional information: To respond to this opportunity please click here: https://ukri.delta-esourcing.com/respond/TB67EP83TB

Award Criteria

CPV Codes

• 38434510 - Cytometers

Indicators

• This is a one-off contract (no recurrence)
• Renewals are not available.

Other Information

** PREVIEW NOTICE, please check Find a Tender for full details. ** The contracting authority considers that this contract may be suitable for economic operators that are small or medium enterprises (SMEs). However, any selection of tenderers will be based solely on the criteria set out for the procurement. For more information about this opportunity, please visit the Delta eSourcing portal at: https://ukri.delta-esourcing.com/tenders/UK-UK-Swindon:-Cytometers./TB67EP83TB To respond to this opportunity, please click here: https://ukri.delta-esourcing.com/respond/TB67EP83TB GO Reference: GO-20221116-PRO-21466229